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Cell Biology: A Laboratory Handbook epub ebook

by Julio E. Celis,Nigel Carter,Kai Simons,J. Victor Small,Tony Hunter,David Shotton

Cell Biology: A Laboratory Handbook epub ebook

Author: Julio E. Celis,Nigel Carter,Kai Simons,J. Victor Small,Tony Hunter,David Shotton
Category: Medicine
Language: English
Publisher: Academic Press; 3 edition (November 30, 2005)
Pages: 2328 pages
ISBN: 0121647307
ISBN13: 978-0121647308
Rating: 4.2
Votes: 490
Other formats: doc mbr lrf lit


Cell Biology: A Laboratory Handbook. J. Victor Small received a P. in 1969 at Kings College, London from where he moved to Denmark to take up a lectureship at Aarhus University, which he held until 1977.

Cell Biology: A Laboratory Handbook. Julio E. Celis, Nigel Carter, Kai Simons, J. Victor Small, Tony Hunter, David Shotton. Thereafter, he became department head in a new institute, the Institute of Molecular Biology, established by the Austrian Academy of Sciences in Salzburg, of which he is the current director. His work has centered on smooth muscle biochemistry and structure and on the mechanism of cell motility.

Cell Biology: A Laboratory Handbook should become the bible of established cell biological methods. -Tony Hunter, Salk Institute, La Jolla, California "The new Handbook is likely to become the standard laboratory manual in cell biology

Cell Biology: A Laboratory Handbook should become the bible of established cell biological methods. -Tony Hunter, Salk Institute, La Jolla, California "The new Handbook is likely to become the standard laboratory manual in cell biology.

Julio E. Celis, David Shotton, Nigel Carter, Tony Hunter, J. Victor Small, Kai Simons.

Techniques are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls.

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This four-volume laboratory manual contains comprehensive state-of-the-art protocols essential for research in the life sciences. Techniques are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls. The important steps and results are beautifully illustrated for further ease of use. This collection enables researchers at all stages of their careers to embark on basic biological problems using a variety of technologies and model systems. This thoroughly updated third edition contains 165 new articles in classical as well as rapidly emerging technologies.

Topics covered include:

Cell and Tissue Culture: Associated Techniques, Viruses, Antibodies, Immunocytochemistry (Volume 1)Organelle and Cellular Structures, Assays (Volume 2)Imaging Techniques, Electron Microscopy, Scanning Probe and Scanning Electron Microscopy, Microdissection, Tissue Arrays, Cytogenetics and In Situ Hybridization, Genomics and Transgenic Knockouts and Knock-down Methods (Volume 3)Transfer of Macromolecules, Expression Systems, Gene Expression Profiling (Volume 4)Indispensable bench companion for every life science laboratoryProvides the latest information on the plethora of technologies needed to tackle complex biological problemsIncludes numerous illustrations, some in full color, supporting steps and results
Reviews (2)
Urtte
It wasn't quite in a format I expected, and I've found it to be helpful. I would like to purchase the next volume.

Kardana
Cell biology techniques have been continuously upgraded since the middle of this century, and future developments seem assured. In the 1990s, alone, new developments have led to improved separations capability, detection instrumentation, and new formulations of serum-free media. Any research institution with faculty and students engaged in cell biology will want to have the most current laboratory guidelines available, and this four-volume, peer-reviewed collection of protocols answers that need. The second edition has benefited from suggestions from practicing scientists, filling in gaps from the first edition.
Perhaps the greatest value of this work is its comprehensiveness. Laboratories and libraries can have detailed guidelines for cell culture, separation, staining, growth, apoptosis, preparation of component parts, assays, antibody production, genetic engineering, microscopy, electrophoresis, and more, all in one source. Need to know how to produce transgenic mice? It's here. How to grow viruses? Prepare nuclei? Stain mitochondria? Use fluorecence microscopy? All here.
Although there are 486 contributors to the 250 articles, uniform structure of articles builds continuity and thus ease of use. Most entries adhere to the five-part template (Introduction, Materials and Instrumentation, Procedures, Comments, and Pitfalls). This last section, "Pitfalls," seems especially valuable, as there is no substitute for experience. The main difference between articles tends to be the use of supportive graphs, photos, and tables, though in general (if such a diverse collection can be spoken of "in general") visuals are at a minimum.

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